Targets of interest are amplified in a multiplex PCR reaction. Primer-dimers, non-specific PCR products, and complex molecular debris are biochemically removed before libraries are barcoded with sample indexes. 

The combination of expert primer design and innovative multiplex PCR-based targeted sequencing and library preparation provides multiplexing capability, low input requirement, high sensitivity, easy-to follow workflow, and cost-effective amplicon sequencing. 

Image by National Cancer Institute