Indexed NGS libraries are prepared from DNA. Solution-based target capture enrichment employs probes designed for optimised coverage and depth to capture target sequences in an NGS library. Based on the typing requirements of different genes. Probes are carefully selected to avoid off-target capture, taking into account relevant genomic events. 

Following hybridisation, a wash step is used to remove unbound ’off-target’ DNA, thus enriching the sequencing library for the regions of interest. The accuracy, simplicity and utility of the hybridization approach are well demonstrated. 

Image by Daniil Kuželev