Next Generation Sequencing
egSEQ Next Generation Sequencing (NGS) library preparation, amplicon and hybridisation capture solutions offer researchers and clinicians insights into specific regions of interest in the genome with unrivalled efficiency and cost-effectiveness.
egSEQ customised targeted sequencing panels are designed and built to suit different panel sizes, target regions, and multiplexing requirements for individual applications and needs.
egSEQ Hybridisation Capture
egSEQ Hybridisation Capture is a solution-based hybridisation capture solution employing synthesised probes to capture target sequences in an NGS library, such as SNPs, InDels, CNVs, SVs, gene fusions and MSI, egSEQ Hybridisation Capture solutions utilise RNA-based probes, ensuring better capture efficiency and depth of coverage with excellent uniformity.
• Ideal for targeting SNV, InDel, CNV, STR, Fusion, Pathogen etc.
• Suitable for large regions of interest
• Optimal depth and uniformity of coverage
• Simple reads to genome alignment
• High sensitivity for variant calling
• Highly specific
egSEQ Amplicon is a multiplex PCR based targeted sequencing solution that uses designed PCR primers to flank genetic targets and specifically amplify regions of interest. After amplification, targets of interest are amplified in a multiplex PCR reaction. Primer-dimers, non-specific PCR products, and complex molecular debris are biochemically removed before libraries are barcoded with sample indexes.
The combination of expert primer design and innovative multiplex PCR-based targeted sequencing and library preparation provides multiplexing capability, low input requirement, high sensitivity, easy-to follow workflow, and cost-effective amplicon sequencing.
Low sample input requirements
Ideal for analysing SNV, insertion and deletion (under 50bp)
Simple and fast workflow
Exceptional on-read target rates
GET IN TOUCH
Our service offerings are fully customisable to suit your research aim, data output requirements, and budget. Get in touch with our team of NGS experts for an initial consultation to discuss your project and what we can offer.