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Whole Exome Sequencing of Endangered Harlequin Frogs


In order to avoid extinction in a quickly changing environment, a species must adapt rapidly to avoid extinction. A group of researchers in Panama used a whole exome sequencing approach on 186 samples of two closely related harlequin frog species (Atelopus varius and Atelopus zeteki). With the aim of testing the hypothesis that some A. varius populations have evolved over a short period of time to become less susceptible to Bd infection. The researchers evaluated the amount and distribution of genetic variation among the historical A. varius and A. zeteki populations, in order to analyse the genetic diversity over time. A set of candidate genes were discovered for Bd resistance and there was some evidence of recent migration and gene flow in one of the largest persisting A. varius populations in Panamá.

As a research-led organisation supplying some of the world's largest and most prestigious institutions, we pride ourselves in providing competitive products with exceptional customer service to scientists and clinicians engaging in cutting-edge discoveries. Targeted sequencing enriches specific genomic regions to allow sequencing reads to be optimised in regions that are of interest, producing enhanced coverage in specific areas with unrivalled cost-effectiveness. egSEQ hybridisation capture targeted panels offer a complete solution from assay design through advanced bioinformatics analysis with different options for targeting genes of interest. Our panels provide targeted sequencing of any gene of interest via next-generation sequencing (NGS), providing information on point mutations, insertions/ deletions (INDELs), copy number variations (CNVs), and gene rearrangements for specific genes of interest.


Byrne, A. Q., Richards‐Zawacki, C. L., Voyles, J., Bi, K., Ibáñez, R., & Rosenblum, E. B. (2021). Whole exome sequencing identifies the potential for genetic rescue in iconic and critically endangered Panamanian harlequin frogs. Global Change Biology, 27(1), 50-70


Image by Muhammad Syafi Al - adam


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